The Oxidation of o-Aminophenols by Cytochrome c and Cytochrome Oxidase
نویسندگان
چکیده
منابع مشابه
The oxidation of o-aminophenols by cytochrome c and cytochrome oxidase. I. Enzymatic oxidations and binding of oxidation products to bovine serum albumin.
The mechanism by which a chemical agent binds to proteins is of considerable interest, particularly since a large number of compounds with carcinogenic activity have been found to bind firmly to cellular proteins at the site of tumor formation. Included in this category are the polycyclic aromatic hydrocarbons (l), the aminoazo dyes (2), N-(2.fluorenyl)acetamide (3), and the nitrogen and the su...
متن کاملStudies on Cytochrome Oxidase. Vi. Kinetics of the Aerobic Oxidation of Ferrocytochrome C by Cytochrome Oxidase.
The rate of reaction can be determined by measuring the rate of either oxygen consumption or ferrocytochrome c oxidation. When the rate of oxygen consumption is measured either manometrically or polarographically, a mixture of a limited amount of cytochrome c and an excess of reducing agent, instead of excess ferrocytochrome c, is used as an electron donor system. In this case the true electron...
متن کاملOxidation of ferrocytochrome c by mitochondrial cytochrome c oxidase.
Attempts to rationalize the kinetics of cytochrome c oxidation catalyzed by solubilized mitochondrial cytochrome c oxidase (ferrocytochrome c:oxygen oxidoreductase, EC 1.9.3.1) have been based on assumptions of productive complex formation (Michaelis-Menten approach). However, the range of substrate concentrations used has not, in general, been sufficient to establish a general rate equation. D...
متن کاملThe Cytochrome C-cytochrome Oxidase Complex
In the preceding paper the oxidation of substrates by “indophenol oxidase” was demonstrated to be a joint action of cytochrome and cytochrome oxidase. It was further shown that with a given amount of oxidase the velocity of hydroquinone oxidation reached a maximum as the amount of added cytochrome was increased. The latter fact immediately suggested the probability that the rapid aerobic oxidat...
متن کاملThe oxidation of o-aminophenols by cytochrome c and cytochrome oxidase. III. 2,3-Flurenoquinone from 2-amino-3-fluorenol and binding of quinonoid oxidation products to bovine serum albumin.
The oxidation of the o-aminophenols, 2-amino-1-fluorenol, 3hydroxy-4-aminobiphenyl, and of o-aminophenol itself by cytochrome c and cytochrome oxidase in vitro has been shown to yield colored oxidation products with characteristic visible absorption spectra (2). The present paper is concerned with the identification of these products by chemical synthesis. The oxidation products of 3-hydroxy-4-...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1960
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)64491-6